Published March 3, 2026
| Version v1
Image
Open
Long-term, high-throughput time-lapse microscopy of mouse stem cells
- 1. Helmholtz Zentrum München
Description
Long-term, high-throughput time-lapse microscopy is a powerful tool for studying the differentiation processes of single cells in unprecedented temporal resolution. A high frequency of bright-field imaging (typically on the scale of a few minutes) ensures that moving single cells and cell divisions can be accurately tracked and used for the construction of cellular genealogies. Additionally, fluorescence imaging (which, owing to cell phototoxicity, is typically only possible when spaced out by intervals of 30 min or more) allows the quantification of molecular lineage markers.
We here provide parts of the data used in Buggenthin et al., Nature Methods 2017, http://dx.doi.org/10.1038/nmeth.4182.
Files
120602PH5_p000x.zip
Files
(40.3 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:2445470da686adb63ec0a5c968e37bb9
|
40.3 GB | Preview Download |
Additional details
Related works
- Is supplement to
- Image: 10.1038/nmeth.4182 (DOI)
Dates
- Available
-
2026-03-03
References
- Buggenthin F, Buettner F, Hoppe PS, Endele M, Kroiss M, Strasser M, et al. Prospective identification of hematopoietic lineage choice by deep learning. Nat Methods [Internet]. 2017 Apr;14(4):403–6. Available from: http://dx.doi.org/10.1038/nmeth.4182